The Caspase-Glo® 3/7 Assay is a luminescent assay that measures caspase-3 and -7 activities in purified enzyme preparations or cultures of adherent or. Description. The Caspase-Glo® 3/7 Assay(a,b) is a homogeneous, luminescent assay that measures caspase-3 and -7 activities. The assay provides a. CellEvent™ Caspase-3/7 Green Detection Reagent is a novel fluorogenic substrate for activated caspase-3/7 that is compatible with both live cell and fixed imaging, with absorption/emission maxima of ~/ nm. Activation of caspase-3 is an essential event during apoptosis.
3/7 activity Caspase
G provides sufficient reagents to perform assays; Cat. G and G, 4, assays. A one-step, plate-based assay for continually monitoring apoptosis progression. Measure caspase-6 activity with this homogeneous, luminescent assay. Measure caspase-8 activity with this homogeneous, luminescent assay.
Measure caspase-9 activity with this homogeneous, luminescent assay. High-performance microplate reader for detecting luminescence, fluorescence and absorbance. An assay to kinetically monitor cytotoxicity up to 72 hours with multiplex capability. A bioluminescent method to kinetically monitor viability in cell culture up to 72 hours. Sensitive detection of cytotoxicity for samples low in cell number, including 3D microtissue cultures. Our website does not fully support your browser.
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Activation of caspase-3 is an essential event during apoptosis, making this an optimized reagent for analysis of apoptotic cells. Furthermore, since wash steps are not necessary for detection, fragile apoptotic cells typically lost during wash steps are preserved. This allows for the flexibility to perform end-point assays and probe for other proteins of interest using immunocytochemistry.
For Research Use Only. Not for use in diagnostic procedures. The treated cells were stained first with 7. C, green fluorescence to detect apoptosis and Hoechst nucleic acid stain Cat. M, pink fluorescence to label mitochondria. Cells were then labeled with Hoechst DIC only and B.
Organotypic cultures of the hippocampus were prepared by standard methods 1, 2. The assay was left to load for one hour prior to imaging. One well was used per experimental group, and each well had five slices. The fluorescence intensity was analyzed with Image J software http: Cells were then labeled with 7.
A Z-score was calculated. Cells were then treated with 0. Cells were then centrifuged for 5 minutes at x g, the supernatant was removed, and cells were resuspended in DPBS.
Lastly, cells were stained with propidium iodide for 30 minutes on ice, then analyzed on an a flow cytometer.
Caspase-Glo® 3/7 Assay Systems
I came across the Caspase Glo 3/7 Assay from Promega. Does anyone have . Day 5: Caspase 3/7 activity of transfected treated/untreated cells. Day 6: . Cell Meter™ Caspase Activity Apoptosis Assay Kitsare designed to monitor cell apoptosis through measuring Caspase activation (Cat No. ). 1. Bioconjug Chem. May 16;29(5) doi: /acs. bioconjchem.8b Epub May 1.